Seton Hall University Organic Chemistry Worksheet

Name:Exp #
Date:
Lab Partner
Title: TLC and flash column chromatography
1. Abstract and Purpose: (2 point).
In this laboratory session, the reduction of fluorenone to fluorenol will be carried out, using
sodium borohydride as reducing agent. The reaction will be monitored by using thin-layer
chromatography with time reactions up to 180 seconds. The crude product will be purified
using flash column chromatography, comparing the Rf of the purified product to that of
the crude product.
2. Balanced equation: (2 point)
4 NaBH4
CH3OH
Fluorenol
Fluorenone
3. Reagent Table (Add more rows when needed) (3 points)
Name
M.W.
(g/mol)
Density*
(g/mL)
M.P.*
(Β°C)
B.P.*
(Β°C)
Amount
(grams
or mL)
Fluorenone
180.21

81-85
342
0.5 g
May cause severe eye
damage and irritation.
Toxic if swallowed.
May cause skin
irritation.
Moles
Hazards/Precautions
Sodium
borohydride
37.83

>300
>400
0.1 g
Methanol
32.04
0.792

65Β°C
10 mL

Flammable, volatile.
Dichloromethane
84.93
1.325

40
6 mL

Flammable, volatile.
Name:
Exp #
Date:
Lab Partner
4. Calculations: Shown each calculation for moles of reagents, limiting reagent,
theoretical yield and percent yield. (4 points)
Calculating moles of reagents:
π‘šπ‘œπ‘™π‘’π‘  π‘œπ‘“ π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘›π‘’ = 0.5 𝑔 Γ—
1 π‘šπ‘œπ‘™
= 0.0028 π‘šπ‘œπ‘™π‘’π‘ 
180.21 𝑔
π‘šπ‘œπ‘™π‘’π‘  π‘œπ‘“ π‘ π‘œπ‘‘π‘–π‘’π‘š π‘π‘œπ‘Ÿπ‘œβ„Žπ‘¦π‘‘π‘Ÿπ‘–π‘‘π‘’ = 0.1 𝑔 Γ—
1 π‘šπ‘œπ‘™
= 0.0026 π‘šπ‘œπ‘™π‘’π‘ 
37.83 𝑔
Determining limiting reagent:
π‘šπ‘œπ‘™π‘’π‘  π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘›π‘’ = 0.1 𝑔 Γ—
1 π‘šπ‘œπ‘™ π‘π‘Žπ΅π»4 4 π‘šπ‘œπ‘™π‘’π‘  π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘›π‘’
Γ—
= 0.011 π‘šπ‘œπ‘™π‘’π‘ 
37.83 𝑔
1 π‘šπ‘œπ‘™ π‘π‘Žπ΅π»4
Excess reagent: sodium borohydride
The limiting reagent is fluorenone
Calculating theoretical yield:
π‘‡β„Žπ‘’π‘Ÿπ‘’π‘œπ‘‘π‘–π‘π‘Žπ‘™ 𝑦𝑖𝑒𝑙𝑑 = 0.0028 π‘šπ‘œπ‘™π‘’π‘  π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘›π‘’ Γ—
1 π‘šπ‘œπ‘™ π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘™
182.22 𝑔
Γ—
1 π‘šπ‘œπ‘™ π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘›π‘’
1 π‘šπ‘œπ‘™
π‘‡β„Žπ‘’π‘Ÿπ‘’π‘œπ‘‘π‘–π‘π‘Žπ‘™ 𝑦𝑖𝑒𝑙𝑑 = 0.51 𝑔 π‘“π‘™π‘’π‘œπ‘Ÿπ‘’π‘›π‘œπ‘™
The theoretical yield is 0.51 g fluorenol
The actual yield is
Calculating Percent yield:
Name:
Exp #
Date:
Lab Partner
Reaction percent yield is
5. Procedure, Observations and Data
β€’
Procedure (4 point)
Observations and Lab Data (4 point)
Six different premade TLC plates are
Report all observations and all data that you collect in the
prepared, drawing the spotting line about 5-7
lab here
mm from the bottom of the plate. Each plate
will be used to monitor the reaction at 0, 15,
30, 60, 120, and 180 s.
β€’
Each mark, in time, is separated by 5 mm.
The spot at time 0 corresponds to fluorenone.
β€’
0.5 g of fluorenone are weighed in a 50 mL
round-bottom flask and 10 mL of ethanol are
added, stirring until all of the solid dissolves.
β€’
Using a micropipette, this solution is
β€œspotted” into the TLC plate in the mark 0 s.
β€’
0.1 g of sodium borohydride are
weighed and added to the solution
above, under constant stirring.
β€’
At each of the designated time intervals
the solution is spotted into to the TLC
plate.
Name:
Exp #
Date:
Lab Partner
β€’
Once all of the solutions are spotted in
the plate, it is eluted using
dichloromethane in a developing
chamber. About 5 mL would suffice, as
long as it does not reach the spots.
β€’
Let the TLC develop and mark the
solvent front, to determine the Rf of
each spot afterwards.
β€’
The reaction mixture is transferred to a
50 mL Erlenmeyer flask and 5 mL of
water are added.
β€’
The solution is cool down in an ice bath
and the obtained solid vacuum filtered.
β€’
The crystals are weighed to determine
the actual yield of the reaction
For column chromatography:
β€’
15 g of silica gel are weighed and
transferred to a 50 mL Erlenmeyer
flask
β€’
15 mL of solvent mixture are
added, forming a slurry mixture.
Name:
Exp #
Date:
Lab Partner
β€’
The slur is carefully added into the
column, using a funnel.
β€’
The excess silica on the walls of the
column is washed down with a
pipette and a little more of solvent.
β€’
The sides of the column are gently
tapped to remove air bubbles.
β€’
A thin layer of sand is added to the
top of the column, eluting a bit of
the solvent until the sand is dry but
not the silica gel.
β€’
0.2 g of the crude product is added
to the top of the column and the
walls of the column washed down
with a pipette and a bit more of
solvent.
β€’
5 mL of solvent mixture is carefully
added to the top of the column and
then slowly drained.
β€’
The fractions eluting from the
column are collected ach 8 mL of
Name:
Exp #
Date:
Lab Partner
solution. Running TLC and
comparing it to the pure product.
6. Conclusions and discussions (4 points)
7. References: (1 point)
8. Image of your lab notebook. (3 point)
I reserve the right to modify points on this template at any time.

Don't use plagiarized sources. Get Your Custom Essay on
Seton Hall University Organic Chemistry Worksheet
Just from $13/Page
Order Essay
Calculate the price
Make an order in advance and get the best price
Pages (550 words)
$0.00
*Price with a welcome 15% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
How it works
Receive a 100% original paper that will pass Turnitin from a top essay writing service
step 1
Upload your instructions
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
Pro service tips
How to get the most out of your experience with Writall
One writer throughout the entire course
If you like the writer, you can hire them again. Just copy & paste their ID on the order form ("Preferred Writer's ID" field). This way, your vocabulary will be uniform, and the writer will be aware of your needs.
The same paper from different writers
You can order essay or any other work from two different writers to choose the best one or give another version to a friend. This can be done through the add-on "Same paper from another writer."
Copy of sources used by the writer
Our college essay writers work with ScienceDirect and other databases. They can send you articles or materials used in PDF or through screenshots. Just tick the "Copy of sources" field on the order form.
Testimonials
See why 20k+ students have chosen us as their sole writing assistance provider
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
Psychology
Awesome Job... you are the best!
Customer 454497, February 18th, 2021
Social Work and Human Services
thanks a bunch
Customer 453933, December 1st, 2020
Other
Amazing writer. Truly the best.
Customer 454983, February 19th, 2022
Nursing
Huge help! Fantastic work!
Customer 454991, October 19th, 2021
Psychology
I just finished reading this work good strong start and conclusion too. In addition follows APA guidelines. Thanks.
Customer 453877, April 18th, 2020
Other
Thank you for the quick response
Customer 454677, April 21st, 2021
Senior Seminar
Great Job!!! Thank you
Customer 453503, April 21st, 2020
Business Studies
great paper!
Customer 454811, January 7th, 2022
Business Studies
great job!
Customer 454811, February 21st, 2022
Other
Perfect as always, thank you!
Customer 452961, July 7th, 2020
fundamentals of intermodal transportation
perfect! thanks!
Customer 454525, March 30th, 2021
Statistics
I got an A. Thanks
Customer 453877, January 6th, 2021
11,595
Customer reviews in total
96%
Current satisfaction rate
3 pages
Average paper length
37%
Customers referred by a friend
OUR GIFT TO YOU
15% OFF your first order
Use a coupon FIRST15 and enjoy expert help with any task at the most affordable price.
Claim my 15% OFF Order in Chat
Live Chat+1(978) 822-0999EmailWhatsApp