Unit 2 lab 1

Column Chromatography• Column Chromatography is another common and useful separation
technique in organic
• chemistry. This separation method involves the same principles as
TLC, but can be applied to separate larger quantities than TLC.
Column chromatography can be used on both a large and small scale.
The applications of this technique are wide reaching and cross many
disciplines including biology, biochemistry, microbiology and
medicine. Many common antibiotics are purified by column
• Column chromatography allows us to separate and collect the compounds
• In this experiment, Column Chromatography (abbreviated CC) will be used
to separate the mixture of ferrocene and acetylferrocene and TLC will be
used to monitor the effectiveness of this separation.
• Choosing a Stationary Phase
• As with TLC, alumina and silica are the two most popular stationary phases
in column chromatography.
• The Column Chromatography more polar sample will be retained on the
stationary phase longer.
• Thus the least polar compound will elute from the column first, followed by
each compound in order of increasing polarity.
• Choosing Solvents
• Solvent systems for use as mobile phases in CC can be determined from previous TLC
experiments, the literature, or experimentally.
• Normally, a separation will begin by using nonpolar or low polarity solvent, allowing the
compounds to adsorb to the stationary phase, then slowly switching the polarity of the solvent to
desorb the compounds and allow them to travel with the mobile phase.
• The polarity of the solvents should be changed gradually.
• On a macroscale, the mixing of two solvents can create heat and crack the column leading to a
poor separation.
• Some typical solvent combinations are ligroin-dichloromethane, hexane-ethyl acetate and
• Often an experimentally determined ratio of these solvents can sufficiently separate most
• Solvents such as methanol and water are normally not used because they can destroy the
integrity of the stationary phase by dissolving some of the silica gel.
• Apparatus
• Columns can be as thin as a pencil to a diameter of several feet in industrial
• They can separate milligram to kilogram quantities of materials. In this
experiment, we will be separating a mixture of approximately 50 mg, so a
small column can be used.
• It is essential to have several clean tared Erlenmeyer flasks, reaction tubes,
beakers, test tubes or vials available to collect the solvent and compounds
as they elute.
• Once you have the general set-up prepared, you can move on to packing
the stationary phase in the column.
• 1. Using gloves, obtain a TLC plate.
• 2. Use a ruler and draw a straight line across the TLC plate exactly 1.0 cm from the
bottom of the plate.
• 3. Draw three hash marks evenly spread across the straight line. Begin assembly of the
chromatography column.
• 4. Obtain a column with a stopcock mechanism and pack the bottom of the column with
a small amount of cotton. Use a glass rod to pack the cotton at the bottom of the
• 5. Fill the bottom of the column with 1.0 cm of sand. Proceed to add 13.0 mL of silica.
Afterwards, securely attach the column to a stand.
• 6. Add another 1.0 cm of sand to the top of the silica layer.
• 7. Using a 50.0 mL beaker, obtain approximately 0.150 g of acetylferrocene and ferrocene
mixture. Add 2.0 mL of dichloromethane to the mixture, ensuring the acetylferrocene
and ferrocene mixture goes into solution.
• 8. Add 10.0 mL of hexane to the column with a collection beaker on the bottom. Add hexane until it is 1.0 cm
from the top of the column.
• 9. Using a pipette, add acetylferrocene and ferrocene mixture. mixture one drop at a time. Then proceed to
add 10.0 mL of hexane.
• 10. Wait until a yellow solution begins to drip from the bottom of the column. Once a yellow solution begins
to drip, place a small flask to collect the solution.
• 11. After all of the yellow residue has left the column, add 10.0 mL of 50:50 hexane-ethyl acetate to the
column. Once orange solution begins to drip from the bottom of the stopcock, place a second small flask for
• 12. After collecting the second solution, there should be two flasks: one orange and one yellow flask. Use a
TLC capillary to spot each solution onto the TLC plate. Place the TLC plate into the TLC jar, allowing the 30:1
mixture to travel up the TLC plate until 2.0 cm from the top of the plate.
• 13. After spotting onto the TLC plate, place the flasks on a hot plate to evaporate. Once evaporated, weigh
the column on a weighing scale. Record the weight.
• 14. Calculate % recovery of ferrocene and acetylferrocene.

Don't use plagiarized sources. Get Your Custom Essay on
Unit 2 lab 1
Just from $13/Page
Order Essay
Calculate the price
Make an order in advance and get the best price
Pages (550 words)
*Price with a welcome 15% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
How it works
Receive a 100% original paper that will pass Turnitin from a top essay writing service
step 1
Upload your instructions
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
Pro service tips
How to get the most out of your experience with Writall
One writer throughout the entire course
If you like the writer, you can hire them again. Just copy & paste their ID on the order form ("Preferred Writer's ID" field). This way, your vocabulary will be uniform, and the writer will be aware of your needs.
The same paper from different writers
You can order essay or any other work from two different writers to choose the best one or give another version to a friend. This can be done through the add-on "Same paper from another writer."
Copy of sources used by the writer
Our college essay writers work with ScienceDirect and other databases. They can send you articles or materials used in PDF or through screenshots. Just tick the "Copy of sources" field on the order form.
See why 20k+ students have chosen us as their sole writing assistance provider
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
Social Work and Human Services
Customer 453933, October 27th, 2020
Criminal Justice
great paper. came fast and exactly what I needed
Customer 455099, November 13th, 2021
Social Work and Human Services
Good job, I will make the necessary adjustments to make it a little more personal to fit my needs.
Customer 453933, September 21st, 2020
Utilized my word provided; synthesized and organized. Including a timely delivery, including revisions. Very pleased.
Customer 455341, May 1st, 2022
Thanks for your help on this. A couple of the references and footnotes needed minor adjustments however no issue to do. Thanks so much!
Customer 455105, November 14th, 2021
fundamentals of intermodal transportation
perfect! thanks!
Customer 454525, March 30th, 2021
Perfect as always, thank you!
Customer 452961, July 7th, 2020
Criminal Justice
Absolutely LOVE the essay I received. I really appreciate it so much.
Customer 454561, February 16th, 2021
Thank you so much!!
Customer 454959, October 22nd, 2021
I would highly recommend this service to anyone struggling with writing. I am good with numbers but not writing. I actually used two services to see which was better. I copy and pasted both instructions the exact same way with customscholars and the other with studybay. Study bay did not follow any of the instructions and even used a different article than I provided not only that study bay i asked for a revised paper due to it not being anything according to my instructions and it is not due for another day which I need it before they can revise it. Customscholars delivered ON time, quicker more efficiently AND even revised a couple things I noticed I wanted to add into the essay. This is my first time using a service like this and I will not go anywhere else!
Customer 455199, December 11th, 2021
The essay was very well formatted and the writer really made it detailed and concise. Followed prompt and had good evidence
Customer 454933, October 5th, 2021
Business Studies
unbelievable work!! outstanding!!!
Customer 454811, January 14th, 2022
Customer reviews in total
Current satisfaction rate
3 pages
Average paper length
Customers referred by a friend
15% OFF your first order
Use a coupon FIRST15 and enjoy expert help with any task at the most affordable price.
Claim my 15% OFF Order in Chat
Live Chat+1(978) 822-0999EmailWhatsApp

Order your essay today and save 20% with the discount code GOLDEN